The SARM1 Fluorogenic Assay Kit (Hydrolase Activity) is designed to measure NAD⁺ cleavage activity in screening and profiling applications. The SARM1 assay kit comes in a convenient 96-well or 384-well format, with enough recombinant human SARM1 enzyme, its substrate N6-etheno-NAD (e-NAD), and SARM1 assay buffer for 100 or 400 enzyme reactions. In addition, the kit includes a SARM1 inhibitor (DSRM-3716) for use as a control inhibitor.

Figure 1: Assay principle.
Hydrolase activity of SARM1 is measured by following the hydrolysis of Etheno-NAD to form Etheno-ADPR + nicotinamide. Etheno-NAD is not fluorescent due to internal quenching. Upon hydrolysis by SARM1, nicotinamide is released, leading to an increase in fluorescence signal directly proportional to the enzymatic activity.