The NFAT Luciferase Reporter Jurkat Cell Line expresses firefly luciferase under the control of the NFAT response elements stably integrated into the Jurkat cell genome. This reporter cell line is designed to monitor T cell activation as well as inhibition by various immune checkpoint inhibitors; it can be used as a control or parental cell line to co-express various immune checkpoint inhibitors, such as PD-1.

This cell line has been functionally validated and responds to thapsigargin, ionomycin, phorbol 12-myristate 13-acetate (PMA), anti-CD3 antibodies, and Dynabeads™ Human T-Activator CD3/CD28. It can be used to measure T cell activation through a variety of TCR activators including TCR activator (anti-CD3ε scFv)/CHO cells (BPS Bioscience #60539) and CD3 x CD19 bispecific antibody (Blinatumomab) in the presence of CD19+ Raji cells.

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Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.