The SiMPLe CHC Antibody Labeling Kit leverages Sortase Mediated Protein Ligation to specifically label recombinant antibodies containing the sequence LPXTG at the C-terminus of the heavy chain (CHC). A diverse number of poly-Glycine (G)_n molecules and labels (e.g. fluorophores, biotin, enzymes, peptides, etc.) are compatible with this kit. The site-specific conjugation ensures that the antigen binding site remains available for target binding and reduces heterogeneity compared to chemical conjugation methods, making it ideal for Antibody-Drug Conjugate (ADC) development and flow cytometry. The kit provides reagents to label 3 x 100 μg of recombinant antibody, as well as purification columns to aid in the removal of excess poly-Glycine (G)_n-label. Anti-HER2-LPETGH₆ and GGGK-FITC are included as positive controls.

Figure 1: Illustration of the mechanism of targeted labeling using the SiMPLe CHC Antibody Labeling Kit (Sortase Mediated Protein Ligation).
Each antibody contains two Sortase recognition sequences, so a maximum of two labels per antibody can be achieved