Casitas B lineage lymphoma (c-Cbl) is a multifunctional protein with ubiquitin E3 ligase activity capable of degrading diverse sets of proteins. c-Cbl is a ubiquitously expressed mammalian protein that plays a vital role in fundamental cellular functions including cell survival, migration, and proliferation. Recent evidence suggests a critical role for c-Cbl in angiogenesis and human solid organ tumors, making it a potential drug target in cancer immunotherapy. The c-CBL TR-FRET Assay Kit is designed to measure c-CBL auto-ubiquitination activity in a homogeneous 384 reaction format. It utilizes Biotin-labeled Ubiquitin and a terbium-labeled anti-c-CBL antibody to complete the TR-FRET pairing. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications.

Figure: Illustration of Assay Principle