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DCAF11 Intrachain TR-FRET Assay Kit

  • 產(chǎn)品型號(hào)：78542
  • 廠商性質(zhì)：生產(chǎn)廠家
  • 更新時(shí)間：2023-11-12
  • 訪  問(wèn)  量：184
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Description

The DCAF11 Intrachain TR-FRET Assay Kit is a sensitive high-throughput screening (HTS) TR-FRET Assay Kit designed to measure DCAF11 auto-ubiquitination activity in a homogeneous 384-reaction format. It utilizes Europium-labeled Ubiquitin (donor) and Cy5-labeled Ubiquitin (acceptor) to complete the TR-FRET pairing. This assay measures poly-ubiquitination since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains formed on DCAF11. As a homogenous assay, it requires no time-consuming washing steps, making it especially suitable for HTS applications as well as real-time analyses.

Figure 1. DCAF11 Intrachain TR-FRET Assay Kit Schematic.

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Synonyms
DDB1- and CUL4-associated factor 11, WD repeat-containing protein 23, WDR23, GL014, PRO2389
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