This recombinant Jurkat cell line was engineered for conditional expression of firefly luciferase. It contains a firefly luciferase gene under the control of a human Interleukin-2 (IL-2) promoter stably integrated into the genome.

The IL-2 Luciferase Reporter Jurkat cell line is suitable to monitor the activation of the IL-2 promoter. This reporter cell line has been functionally validated using various agonists such as phorbol 12-myristate 13-acetate (PMA) in combination with ionomycin, an anti-human CD3 antibody, anti-human CD3/CD28 Dynabeads, and an anti-CD19/anti-CD3 bispecific antibody. It has also been validated in a co-culture assay with our TCR activator CHO cell line.

Figure 1. Illustration of IL-2 promoter region with representative transcription factor binding site.

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