The IDO1 Cellular Activity QuickDetect™ Supplements are designed to complement the hIDO1-HEK293 Recombinant Cell Line (BPS Cat. #60532) or other IDO1- expressing cell lines. This kit contains the cell culture medium supplements necessary for activation of IDO1 and for the analysis and detection of tryptophan 2,3 dioxygenase (IDO1)- catalyzed conversion of L-tryptophan (L-Trp) to Kynurenine (Kyn). The supplements and the detection reagents, when used as described, allow for indirect measurement of Kyn levels by analyzing absorption at 480 nm.